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KMID : 0613820060160020315
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Journal of Life Science
2006 Volume.16 No. 2 p.315 ~ p.322
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Effect of FS11052, an Inhibitor of Exocytosis, on Neurite Extension in Rat Hippocampal Neurons and PC12 Cells
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Lee Yun-Sik
Kim Dong-Sup
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Abstract
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FS11052, a novel microbial metabolite from Streptomyces spp. was identified as a small molecular substance and shown inhibition activities for the release of neurotransmitter from rat hippocampal neuron and PC12 cells. FS11052 is an inhibitor of tritiated norepinephrine ([³H]-NE) release in high K+ buffer solution containing ionomycin, indicating that FS11052 inhibits neurotransmitter release after the influx of Ca2+ ions. When examined the effect of FS11052 on glucuronidase release from guinea pig neutrophils, FS11052 inhibited glucuronidase release: when treated with 5 ㎍/㎖ of FS11052, which was not induced cellular cytotoxicity. The fact that the glucuronidase release in neutrophil and norepinephrine release in neuron was inhibited suggests the similarity in the locations and the mechanisms of FS11052 action targets. When treated with 5 ㎍/㎖ of FS11052, [³H]-NE release and neurite extension for both rat hippocampal neurons and PC12 cells were prevented. These observations of FS11052 functioning as an inhibitor of neurotransmitter release suggest that FS11052 has an important role in synaptic transmission in neuron.
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KEYWORD
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Neuron, exocytosis, inhibitor, neurophil, glucuronidase
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